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magnetic beads conjugated using a human anti–argonaute 2 (ago2) antibody - by Bioz Stars, 2026-09
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magnetic beads conjugated to either a human anti-argonaute 2 (ago2) antibody  (Millipore)
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human anti argonaute2 anti ago2 antibody  (Proteintech)
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Fig. 4 MIR4435-2HG targets miR-1252-5p in pancreatic cancer cells.(A) Predicted binding sites of MIR4435-2HG on the 3’-UTR of miR-1252-5p, along with results from the dual-luciferase reporter assay, showing a significant reduction in luciferase activity in cells transfected with wild-type miR-1252-5p luciferase vectors. (B) RNA pull-down assay demonstrating the interaction between MIR4435-2HG and miR-1252-5p in BxPC-3 and PANC-1 cells. (C) RIP assay showing the binding efficiency of MIR4435-2HG and miR-1252-5p to <t>AGO2</t> protein. (D) RT-qPCR analysis of miR-1252-5p expression levels in HPNE, BxPC-3, and PANC-1 cells. *p<0.05, **p<0.01, ***p<0.001
Human Anti Argonaute2 Anti Ago2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human argonaute 2 (ago2) antibody  (Millipore)
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Millipore anti-human argonaute 2 (ago2) antibody
Fig. 4 MIR4435-2HG targets miR-1252-5p in pancreatic cancer cells.(A) Predicted binding sites of MIR4435-2HG on the 3’-UTR of miR-1252-5p, along with results from the dual-luciferase reporter assay, showing a significant reduction in luciferase activity in cells transfected with wild-type miR-1252-5p luciferase vectors. (B) RNA pull-down assay demonstrating the interaction between MIR4435-2HG and miR-1252-5p in BxPC-3 and PANC-1 cells. (C) RIP assay showing the binding efficiency of MIR4435-2HG and miR-1252-5p to <t>AGO2</t> protein. (D) RT-qPCR analysis of miR-1252-5p expression levels in HPNE, BxPC-3, and PANC-1 cells. *p<0.05, **p<0.01, ***p<0.001
Anti Human Argonaute 2 (Ago2) Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4 MIR4435-2HG targets miR-1252-5p in pancreatic cancer cells.(A) Predicted binding sites of MIR4435-2HG on the 3’-UTR of miR-1252-5p, along with results from the dual-luciferase reporter assay, showing a significant reduction in luciferase activity in cells transfected with wild-type miR-1252-5p luciferase vectors. (B) RNA pull-down assay demonstrating the interaction between MIR4435-2HG and miR-1252-5p in BxPC-3 and PANC-1 cells. (C) RIP assay showing the binding efficiency of MIR4435-2HG and miR-1252-5p to AGO2 protein. (D) RT-qPCR analysis of miR-1252-5p expression levels in HPNE, BxPC-3, and PANC-1 cells. *p<0.05, **p<0.01, ***p<0.001

Journal: Journal of translational medicine

Article Title: Long non-coding RNA MIR4435-2HG modulates pancreatic cancer stem cells and chemosensitivity to gemcitabine by targeting the miR-1252-5p/STAT1.

doi: 10.1186/s12967-025-06128-8

Figure Lengend Snippet: Fig. 4 MIR4435-2HG targets miR-1252-5p in pancreatic cancer cells.(A) Predicted binding sites of MIR4435-2HG on the 3’-UTR of miR-1252-5p, along with results from the dual-luciferase reporter assay, showing a significant reduction in luciferase activity in cells transfected with wild-type miR-1252-5p luciferase vectors. (B) RNA pull-down assay demonstrating the interaction between MIR4435-2HG and miR-1252-5p in BxPC-3 and PANC-1 cells. (C) RIP assay showing the binding efficiency of MIR4435-2HG and miR-1252-5p to AGO2 protein. (D) RT-qPCR analysis of miR-1252-5p expression levels in HPNE, BxPC-3, and PANC-1 cells. *p<0.05, **p<0.01, ***p<0.001

Article Snippet: Cell lysates were prepared and incubated in RIP buffer with magnetic beads conjugated to either a human anti-Argonaute2 (anti-Ago2) antibody (Proteintech) or IgG control.

Techniques: Binding Assay, Luciferase, Reporter Assay, Activity Assay, Transfection, Pull Down Assay, Quantitative RT-PCR, Expressing